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mouse il-12 primer  (Qiagen)
90
Qiagen mouse il-12 primer
Mouse Il 12 Primer, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse il-12 primer/product/Qiagen
Average 90 stars, based on 1 article reviews
mouse il-12 primer - by Bioz Stars, 2026-02
90/100 stars
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mouse il-12 p40 primer pairs (amplicon size, 396 bp)  (Thermo Fisher)
90
Thermo Fisher mouse il-12 p40 primer pairs (amplicon size, 396 bp)
A, sequence of wild type <t>IL-12</t> <t>p40</t> promoter encompassing the CACCC element and its CACCC element mutant oligonucleotides used in EMSA, together with other identified functional cis-elements in human IL-12 p40 promoter. B, left panel shows a supershift EMSA in which lane 1 shows probe only control and lanes 2 (wild type probe) and lane 6 (mutant probe) show probe binding in absence of antisera (no antibodies). Nuclear extracts (NE) were incubated with antisera against human Kruppel-like factor family members including 1 μl of EKLF (lane 3), BKLF (lane 4), or KLF8 (lane 5). Note that only EKLF antisera were able to supershift the CACCC-binding protein. Right panel, analysis of binding site specificity through competition and mutation EMSAs. Lanes with competition EMSA reaction had cold wild type (WT) or mutant oligonucleotides in molar excess from 4- to 256-fold (in 4-fold increments) over the 32P-labeled wild type oligonucleotides before protein-DNA interaction reactions. The arrows indicate the specific CACCC element binding product. The EMSA is a representative of three similar experiments.
Mouse Il 12 P40 Primer Pairs (Amplicon Size, 396 Bp), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse il-12 p40 primer pairs (amplicon size, 396 bp)/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
mouse il-12 p40 primer pairs (amplicon size, 396 bp) - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
primers for mouse il-12/p35  (SuperArray Bioscience Corporation)
90
SuperArray Bioscience Corporation primers for mouse il-12/p35
A, sequence of wild type <t>IL-12</t> <t>p40</t> promoter encompassing the CACCC element and its CACCC element mutant oligonucleotides used in EMSA, together with other identified functional cis-elements in human IL-12 p40 promoter. B, left panel shows a supershift EMSA in which lane 1 shows probe only control and lanes 2 (wild type probe) and lane 6 (mutant probe) show probe binding in absence of antisera (no antibodies). Nuclear extracts (NE) were incubated with antisera against human Kruppel-like factor family members including 1 μl of EKLF (lane 3), BKLF (lane 4), or KLF8 (lane 5). Note that only EKLF antisera were able to supershift the CACCC-binding protein. Right panel, analysis of binding site specificity through competition and mutation EMSAs. Lanes with competition EMSA reaction had cold wild type (WT) or mutant oligonucleotides in molar excess from 4- to 256-fold (in 4-fold increments) over the 32P-labeled wild type oligonucleotides before protein-DNA interaction reactions. The arrows indicate the specific CACCC element binding product. The EMSA is a representative of three similar experiments.
Primers For Mouse Il 12/P35, supplied by SuperArray Bioscience Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primers for mouse il-12/p35/product/SuperArray Bioscience Corporation
Average 90 stars, based on 1 article reviews
primers for mouse il-12/p35 - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
primers for p35 and p40 for mouse il-12  (Sangon Biotech)
90
Sangon Biotech primers for p35 and p40 for mouse il-12
A, sequence of wild type <t>IL-12</t> <t>p40</t> promoter encompassing the CACCC element and its CACCC element mutant oligonucleotides used in EMSA, together with other identified functional cis-elements in human IL-12 p40 promoter. B, left panel shows a supershift EMSA in which lane 1 shows probe only control and lanes 2 (wild type probe) and lane 6 (mutant probe) show probe binding in absence of antisera (no antibodies). Nuclear extracts (NE) were incubated with antisera against human Kruppel-like factor family members including 1 μl of EKLF (lane 3), BKLF (lane 4), or KLF8 (lane 5). Note that only EKLF antisera were able to supershift the CACCC-binding protein. Right panel, analysis of binding site specificity through competition and mutation EMSAs. Lanes with competition EMSA reaction had cold wild type (WT) or mutant oligonucleotides in molar excess from 4- to 256-fold (in 4-fold increments) over the 32P-labeled wild type oligonucleotides before protein-DNA interaction reactions. The arrows indicate the specific CACCC element binding product. The EMSA is a representative of three similar experiments.
Primers For P35 And P40 For Mouse Il 12, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primers for p35 and p40 for mouse il-12/product/Sangon Biotech
Average 90 stars, based on 1 article reviews
primers for p35 and p40 for mouse il-12 - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier

Image Search Results


A, sequence of wild type IL-12 p40 promoter encompassing the CACCC element and its CACCC element mutant oligonucleotides used in EMSA, together with other identified functional cis-elements in human IL-12 p40 promoter. B, left panel shows a supershift EMSA in which lane 1 shows probe only control and lanes 2 (wild type probe) and lane 6 (mutant probe) show probe binding in absence of antisera (no antibodies). Nuclear extracts (NE) were incubated with antisera against human Kruppel-like factor family members including 1 μl of EKLF (lane 3), BKLF (lane 4), or KLF8 (lane 5). Note that only EKLF antisera were able to supershift the CACCC-binding protein. Right panel, analysis of binding site specificity through competition and mutation EMSAs. Lanes with competition EMSA reaction had cold wild type (WT) or mutant oligonucleotides in molar excess from 4- to 256-fold (in 4-fold increments) over the 32P-labeled wild type oligonucleotides before protein-DNA interaction reactions. The arrows indicate the specific CACCC element binding product. The EMSA is a representative of three similar experiments.

Journal:

Article Title: Activation and Repression of Interleukin-12 p40 Transcription by Erythroid Kruppel-like Factor in Macrophages *

doi: 10.1074/jbc.M400320200

Figure Lengend Snippet: A, sequence of wild type IL-12 p40 promoter encompassing the CACCC element and its CACCC element mutant oligonucleotides used in EMSA, together with other identified functional cis-elements in human IL-12 p40 promoter. B, left panel shows a supershift EMSA in which lane 1 shows probe only control and lanes 2 (wild type probe) and lane 6 (mutant probe) show probe binding in absence of antisera (no antibodies). Nuclear extracts (NE) were incubated with antisera against human Kruppel-like factor family members including 1 μl of EKLF (lane 3), BKLF (lane 4), or KLF8 (lane 5). Note that only EKLF antisera were able to supershift the CACCC-binding protein. Right panel, analysis of binding site specificity through competition and mutation EMSAs. Lanes with competition EMSA reaction had cold wild type (WT) or mutant oligonucleotides in molar excess from 4- to 256-fold (in 4-fold increments) over the 32P-labeled wild type oligonucleotides before protein-DNA interaction reactions. The arrows indicate the specific CACCC element binding product. The EMSA is a representative of three similar experiments.

Article Snippet: Mouse IL-12 p40 primer pairs (amplicon size, 396 bp), Classic 18S primer™ pairs (amplicon size, 488 bp), Classic II 18S primer™ pairs (amplicon size, 324 bp), and the corresponding competimor primer pairs were purchased from Ambion.

Techniques: Sequencing, Mutagenesis, Functional Assay, Binding Assay, Incubation, Labeling

A, detection of endogenous and overexpressed EKLF in RAW264.7 cells by Western blots. Total proteins from each culture were immunoblotted with anti-actin as a control. B, the normalized fold induction obtained from RAW264.7 cells transfected with either wild type human IL-12 p40 (WT Hp40-luc.) or its CACCC element mutant form (Mutant Hp40-luc.) is shown. Transfection efficiency was normalized against co-transfected CMV-β-galactosidase plasmids. The mean value of the basal unstimulated wild type IL-12 p40 promoter activity was set at 1. The transcriptional activity was expressed as fold induction from the wild type IL-12 p40 promoter activity. Error bars represent the S.D. for n = 6.

Journal:

Article Title: Activation and Repression of Interleukin-12 p40 Transcription by Erythroid Kruppel-like Factor in Macrophages *

doi: 10.1074/jbc.M400320200

Figure Lengend Snippet: A, detection of endogenous and overexpressed EKLF in RAW264.7 cells by Western blots. Total proteins from each culture were immunoblotted with anti-actin as a control. B, the normalized fold induction obtained from RAW264.7 cells transfected with either wild type human IL-12 p40 (WT Hp40-luc.) or its CACCC element mutant form (Mutant Hp40-luc.) is shown. Transfection efficiency was normalized against co-transfected CMV-β-galactosidase plasmids. The mean value of the basal unstimulated wild type IL-12 p40 promoter activity was set at 1. The transcriptional activity was expressed as fold induction from the wild type IL-12 p40 promoter activity. Error bars represent the S.D. for n = 6.

Article Snippet: Mouse IL-12 p40 primer pairs (amplicon size, 396 bp), Classic 18S primer™ pairs (amplicon size, 488 bp), Classic II 18S primer™ pairs (amplicon size, 324 bp), and the corresponding competimor primer pairs were purchased from Ambion.

Techniques: Western Blot, Transfection, Mutagenesis, Activity Assay

Multiplex RT-PCRs were performed to detect IL-12 p40 mRNA in EKLF-transfected cells enriched through cell sorting. The experiment shown is a representative of three similar experiments.

Journal:

Article Title: Activation and Repression of Interleukin-12 p40 Transcription by Erythroid Kruppel-like Factor in Macrophages *

doi: 10.1074/jbc.M400320200

Figure Lengend Snippet: Multiplex RT-PCRs were performed to detect IL-12 p40 mRNA in EKLF-transfected cells enriched through cell sorting. The experiment shown is a representative of three similar experiments.

Article Snippet: Mouse IL-12 p40 primer pairs (amplicon size, 396 bp), Classic 18S primer™ pairs (amplicon size, 488 bp), Classic II 18S primer™ pairs (amplicon size, 324 bp), and the corresponding competimor primer pairs were purchased from Ambion.

Techniques: Multiplex Assay, Transfection, FACS